Composite

Part:BBa_K4343079:Design

Designed by: Yue Shu   Group: iGEM22_NNU-China   (2022-10-09)


pUC-HUH-IntC-PaDes12


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3344
    Illegal XhoI site found at 3377
    Illegal XhoI site found at 8005
    Illegal XhoI site found at 8921
    Illegal XhoI site found at 9212
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3532
    Illegal AgeI site found at 1422
    Illegal AgeI site found at 1521
    Illegal AgeI site found at 1882
    Illegal AgeI site found at 4585
    Illegal AgeI site found at 4946
    Illegal AgeI site found at 6088
    Illegal AgeI site found at 7310
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1425
    Illegal BsaI site found at 2062
    Illegal BsaI site found at 5126
    Illegal BsaI site found at 5663
    Illegal BsaI site found at 6091
    Illegal BsaI site found at 7313
    Illegal BsaI.rc site found at 1419
    Illegal BsaI.rc site found at 6085
    Illegal BsaI.rc site found at 7307
    Illegal BsaI.rc site found at 7807
    Illegal BsaI.rc site found at 7817
    Illegal BsaI.rc site found at 9235
    Illegal SapI site found at 8733
    Illegal SapI.rc site found at 1787
    Illegal SapI.rc site found at 4851


Design Notes

None


Source

from Parietiestrium sp.

References